![]() ![]() Perform transfer in tank apparatus placed in ice or a temperature-controlled (4☌) room. ![]() Reduce current, and increase transfer time to compensate.High-intensity transfers in tank blotting sample can cause buffer heating, leading to bubble formation ensure that buffer remains cool.Visualize total protein on gels and blots using Bio-Rad’s Stain-Free Gels featuring our proprietary Stain-Free Technology).To verify protein transfer, stain the membrane with Ponceau S after blotting.To determine if there is residual, untransferred protein remaining on the gel, use a total protein stain on the gel after transfer, e.g., Coomassie, colloidal gold, or SYPRO Ruby.For example, Coomassie and colloidal gold are not compatible with downstream steps (see Bio-Rad Protein Stains and the Protein Stain Selection Guide). ![]() If planning to use the blot in downstream steps, make sure that your stain can be removed or is compatible with antibody detection.
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